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KMID : 0624620080410010068
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BMB Reports
2008 Volume.41 No. 1 p.68 ~ p.71
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Characterization of flavone synthase I from rice
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Lee Yoon-Jung
Kim Jeong-Ho
Kim Bong-Gyu
Lim Yoong-Ho
Ahn Joong-Hoon
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Abstract
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Flavones are synthesized from flavanones through the action of flavone synthases (FNSs). There are two FNSs, FNS I and II. FNS I is a soluble dioxygenase present in members of the Apiaceae family and FNS II is a membrane bound cytochrome P450 enzyme that has been identified in numerous plant species. In this study, we cloned OsFNS I-1 from rice by RTPCR, expressed it in E. coli, and purified the recombinant protein. By NMR analysis, we found that OsFNS I-1 converted the flavanone (2S)-naringenin into the flavone, apigenin. Moreover, we found that the cofactors oxoglutarate, FeSO4, ascorbate and catalase are required for this reaction. OsFNS I-1 encodes a flavone synthase I. This is the first type I FNS I found outside of the Apiaceae family. [BMB reports 2008; 41(1): 68-71]
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KEYWORD
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Apigenin, Flavone synthase, Naringenin, Oryza sativa
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